Stromal cell-derived extracellular matrix promotes the proliferation and retains the osteogenic differentiation capacity of mesenchymal stem cells on three-dimensional scaffolds
News from 3DCellCulture.com (RSS feed)
Mon, 07 Jul 2014 18:17:21 EST
To date, expansion of bone marrow-derived mesenchymal stem cells (MSCs) is typically carried out on two-dimensional (2D) tissue culture plastic. Since this 2D substratum is very different from the physiological situation, MSCs gradually lose their unique multipotent properties during expansion. Recently, the role of the extracellular matrix (ECM) microenvironment ("niche") in facilitating and regulating stem cell behavior in vivo has been elucidated. As a result, investigators have shifted their...
Screening candidate metastasis-associated genes in three-dimensional HCC spheroids with different metastasis potential
Mon, 07 Jul 2014 18:17:19 EST
PURPOSE: Previously, we have established a tissue-like HCC spheroid which better mirrors the biological features of tumorigenesis and metastasis. This study was to find out metastasis-associated genes between two 3D HCC spheroids with different metastasis potential using comparative PCR arrays. MATERIALS AND METHODS: Two HCC spheroids derived from high-metastatic MHCC97H cells and low-metastatic Hep3B cells were formed respectively in a rotating wall vessel bioreactor after 3D culture for 15 day...
Electrohydrodynamic jet process for pore-structure-controlled 3D fibrous architecture as a tissue regenerative material: Fabrication and cellular activities
Mon, 07 Jul 2014 18:17:17 EST
In this study, we propose a new scaffold fabrication method, "direct electro-hydrodynamic jet process," using the initial jet of an electrospinning process and ethanol media as a target. The fabricated three-dimensional (3D) fibrous structure was configured with multi-layered micro-sized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes (ECMs). The fabrication of the structure was highly dependent on various processing parame...
Enhanced cellular activities of polycaprolactone/alginate-based cell-laden hierarchical scaffolds for hard tissue engineering applications
Mon, 07 Jul 2014 18:17:16 EST
Biomedical scaffolds have been widely investigated because they are essential for support and promotion of cell adhesion, proliferation and differentiation in three-dimensional (3D) structures. An ideal scaffold should be highly porous to enable efficient nutrient and oxygen transfer and have a 3D structure that provides optimal micro-environmental conditions for the seeded cells to obtain homogeneous growth after a long culture period. In this study, new hierarchical osteoblast-like cell (MG-63...
Engineered microenvironments provide new insights into ovarian and prostate cancer progression and drug responses
Mon, 07 Jul 2014 18:17:14 EST
Tissue engineering technologies, which have originally been designed to reconstitute damaged tissue structure and function, can mimic not only tissue regeneration processes but also cancer development and progression. Bioengineered approaches allow cell biologists to develop sophisticated experimentally and physiologically relevant cancer models to recapitulate the complexity of the disease seen in patients. Tissue engineering tools enable three-dimensionality based on the design of biomaterials...