Compared to traditional fractionation techniques, advantages of the PF2D chromatographic approach include:
- High loading capacity without band distortion
- Improved detection of low-abundance species
- Membrane or hydrophobic proteins detection
- Enhanced reproducibility
- Contamination-free liquid flow path
Subsequent mass spectrometry analysis is easily coupled to the final fraction collector for MALDI or SELDI analysis of intact proteins or tryptic digestion followed by MALDI or ESI and peptide fingerprint mapping.