Faculty Fellow, Bradstreet Chair in Bioanalytical
Co-Chair, Trans-NIH Alliance of Glycobiologists for
Detection of Cancer and Cancer Risk
Editor-in-Chief, Journal of Proteome Research (ACS)
1970 PhD, Adelaide University, South Australia
1966 BSc, Adelaide University, South Australia
The mission of Dr. Hancock's research program is the study of Glycoproteomics and glycans associated with cancer and infectious disease. Our studies involve the analysis of candidate biomarkers from both patient tissue and blood samples and we use panels of lectins (sugar binding proteins) to isolate and detect changes in glycan composition in disease. Current active area include breast and renal cancer and study of the AIDS virus. The second area of research is the study of biotechnology protein drugs which is an area which requires that latest in mass spectrometry technology especially with the emergence of biogeneric drugs. We are currently analyzing growth hormone, interferon and antibody drugs and developing new analytical approaches for study variants caused by changes in disulfides, glycosylation as well as degradative reactions such as oxidation, deamidation and aggregation.
Recent Invited Lectures
(1) HPLC 2009, June 28th to July 2nd, 2009, Dresden, Germany, "The Role of HPLC in the Discovery of Glycoprotein (Glycan) Markers for the Early Detection of Cancer".
(2) Clinical and Translational Research on Cancer: Glycomics Applications, March 24th to 27th, Toba, Japan, "Glycoprotein Markers for the Early Detecion of Cancer".
(3) 33rd International Symposium on HPLC,
Dec. 2 - 5, 2008, Kyoto, Japan, "The Use of Plasma Proteomics to Study".
(4) 2008 Eastern Asian Glycoscience Symposium, Nov. 28-29, Daejeon, Korea, " The Use of Plasma Glycoproteomics for the Early Detection of Disease".
(5) 30th European Peptide Symposium, Helsinki, Finland, Aug. 31st to Sept. 5, "The Use of Plasma Proteomics to Study Disease Pathogenesis"th, 2008.
(6) HUPO 7th World Congress, Aug. 16th to 20th , 2008, Amsterdam, Netherlands, "The Use of Plasma Proteomics to Study Disease Pathogenesis".
(7) HPLC 2008, May 11th to 16th, 2008, Baltimore, MD, "Signal Amplification in Clinical Proteomics".
Recent Awards (Partial Listing)
(1) President, US HUPO, 2008
(2) Randolph T. Major Memorial Lecturer (R.T.Major Lecture Series, Dept. of Chem., Univ. of Connecticut, 2004)
(3) ACS Award in Separation Science (ACS
National Meeting, 2003)
202 scientific publications, plus 7 books
and 21 patents.
Complete List of Publications>>
Current Pubmed search "W.S. Hancock">>
Selected Recent Publications:
(1) Taylor, A.D., Hancock, W.S., Hincapie, M., Taniguchi, N. and Hanash, S.M., "Towards an Integrated Proteomic and Glycomic Approach to Finding Cancer Biomarkers", Genome Med., Jun 4, 1(6):57, (2009) [Epub ahead of print]. (pubmed)
(2) Bell, A.W., et al,"A HUPO Test Sample Study Reveals Common Problems in Mass Spectrometry-Based Proteomics", Nat Methods, May 17, (2009) [Epub ahead of print]. (pubmed)
(3) Jiang, H., Wu, S-L., Karger,B.L. and Hancock, W.S., "Mass Spectrometric Analysis of Innovator, Counterfeit, and Follow-On Recombinant Human Growth Hormone", Biotechnol. Prog., Jan.-Feb., 25(1), 207-18 (2009). (pubmed)
(4) Zheng, X., Wu, S-L., Hincapie, M. and Hancock, W.S.,"Study of the Human Plasma Proteome of Rheumatoid Arthritis", J. Chromatogr. A, 1216(16), 3538-45 (2009). (pubmed) .
(5) Wu, S-L., Jiang, H., Lu, Q., Dai, S., Hancock, W.S. and Karger, B.L., "Mass Spectrometric Determination of Disulfide Linkages in Recombinant Therapeutic Proteins Using Online LC-MS with Electron-Transfer Dissociation", Anal. Chem., 81, 1, 112-122 (2009).(link)
(6) Ochoa, C.D., Baker, H., Hasak, S., Matyal, R., Salam, A., Hales, C.A., Hancock, W.S. and Quinn, D.A., "Cyclic Stretch Affects Pulmonary Endothelial Cell Control of Pulmonary Smooth Muscle Cell Growth", Am. J. Respir. Cell Mol. Biol. 1, 105-112 (2008). (pubmed)
(7) Pandey, A., et. al., (Hancock W.S.) "Human Proteinpedia enables sharing of human protein data", Nat Biotechnol. , Feb;26(2):164-7 (2008).(pubmed)
(8) Kullolli, M., Hancock, W.S., Hincapie, M., "Preparation of a high-performance multi-lectin affinity chromatography (HP-M-LAC) adsorbent for the analysis of human plasma glycoproteins", J, Sep Sci. (14):2733-9 (2008).(pubmed)
(9) Lu, C., Murugesan, N.. Macdonald, J. A, Wu, S. L., Pachter, J. and Hancock, W. S., "Analysis of Mouse Brain Microvascular Endothelium Using Immuno-Laser Capture Microdissection Coupled to a Hybrid LTQ-FT MS Proteomics Platform”, Electrophoresis, 2689-95 (2008).(pubmed)
(10) Ralin, D.W., Dultz, S.C., Silver, J.E., Kullolli, M., Hancock, W.S. and Hincapie, M., "Kinetic Analysis of Glycoprotein-lectin Interactions by Label- Free Internal Reflection”, Clin. Proteomics, epub, 18 July (2008).
(11) Plavina, T., Hincapie, M., Wakshull, E., Subramanyam M., and Hancock, W.S., “Increased Levels of Cytoskeletal and Ca2+-Binding Proteins and their Peptides in Plasma of Psoriasis Patients”, Clin. Chem., 54,1805-1814 (2008).(pubmed)
(12) Dayarathna, M.K., Hancock, W.S. and Hincapie, M., "A Two-Step Fractionation Approach for Plasma Proteomics Using Immunodepletion of Abundant Proteins and Multi-lectin Affinity Chromatography (M-LAC): Application to the Analysis of Obesity, Diabetes and Hypertension,", J. Sep. Science, 1156-1166 (2008).(pubmed)
(13) Orazine, C. I., Hincapie, M., Hancock, W.S., Hattersley, M., and Hanke, J., "A Proteomic Analysis of the Plasma Glycoproteins of a MCF-7 Mouse Xenograft : A Model System for the Detection of Tumor Markers", J. Prot. Res., 7, 1542-54 (2008). (pubmed)
(14) Wu, S.-L., Gu, Y., Hanlon, D., Meyer, J., Linder, J., Hancock, W.S., and Karger, B.L., "Proteomic Analysis of High-Grade Dysplastic Cervical Cells Obtained From ThinPrep® Slides Using Laser Capture Microdissection and Mass Spectrometry", J. Proteome Res., 6, 4256-68 (2007).
(15) Plavina, T., Wakeshull, E., Hancock, W.S., Hincapie, M., "Combination of abundant protein depletion and multi-lectin affinity chromatography (M-LAC) for plasma protein biomarker discovery", J. Proteome Res., 662-671 (2007).
(16) Zheng, X., Baker, H., and Hancock, W. S., "Analysis of the Low Molecular Weight Serum Peptidome Using Ultrafiltration and a Hybrid Ion Trap-Fourier Transform Mass Spectrometer", J. Chromatogr., 1120, 173-184 (2006).
(17) Plymoth, A., Yang, Z., Lofdahl, C. G., Ekberg-Jansson, A., Dahlback, M., Fehniger, T. E., Marko-Varga, G., Hancock, W. S., "Rapid Proteome Analysis of Bronchoalveolar Lavage Samples of Lifelong Smokers and Never-Smokers by Micro-Scale Liquid Chromatography and Mass Spectrometry", Clin. Chem., 52, 671-679 (2006).
(18) Wang, Y., Wu, S- L. and Hancock, W. S., "Approaches to the Study of N-linked Glycoproteins in Human Plasma Using Lectin Affinity Chromatography and Nano-HPLC Coupled to Electrospray Linear Ion Trap-Fourier Transform Mass Spectrometry (LTQ/FTMS)", Glycobiology, 16, 514-523 (2006).
(19) Wang, Y., Wu, S- L. and Hancock, W. S., "Monitoring of Glycoprotein Products in Cell Culture Lysates Using Lectin Affinity Chromatography and Capillary-HPLC Coupled to Electrospray Linear Ion Trap - Fourier Transform Mass Spectrometry LTQ/FTMS)", Biotechnology Progress, 22, 873-880 (2006).
(20) Zheng, X., Wu, S- L., and Hancock, W. S., "Glycation of Interferon-beta-1b and human serum albumin in a lyophilized glucose formulation", Int. J. Pharmaceutics, 322, 136-145 (2006).
(21) Yang, Z., Harris, L. E., Palmer-Toy, D. E. and Hancock, W. S., "Characterization of Multiple Glycoprotein Biomarker Candidates in Serum from Breast Cancer Patients Using Multi-lectin Affinity Chromatography (M-LAC)", Clin. Chem., 52, 1897-1905 (2006).
(22) Wang, Y., Dai, D., Sadee, W. and Hancock, W.S., "A Pharmacoproteomics Study of the Cancer Cell Line EKVX", Mol. Pharmaceutics, 5, 566-578 (2006).
(23) Zheng, X., Baker, H., Hancock, W. S., Fawaz, F., McCaman, M. and Pungor, E., "Proteomic Analysis for the Assessment of Different Lots of Fetal Bovine Serum as a Raw Material for Cell Culture", Biotechnology Progress, 5, 1294-1300 (2006).
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